By Rosalyn D. Blumenthal
A state-of-the artwork selection of effortlessly reproducible laboratory equipment for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in person tumors. Chemosensitivity, quantity 1: In Vitro Assays offers a panel of sixteen in vitro measures of chemosensitivity in adherent and non-adherent cells for unmarried brokers and mixtures of brokers. as well as immunohistochemical and imaging techniques, those assays comprise clonogenic, colorimetric, fluorometric, and physiological assays. Highlights comprise photograph research to evaluate drug sensitivity, excessive throughput methods utilizing eco-friendly fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay utilized in biotechnology. A spouse quantity, quantity 2: In Vivo versions, Imaging, and Molecular Regulators, presents protocols for classifying tumors into reaction different types and customizing chemotherapy regimens to person sufferers.
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Extra info for Chemosensitivity: Volume I: In Vitro Assays (Methods in Molecular Medicine)
Med. 15, 471–486. 34. , et al. (2002) Increased uptake of the apoptosis imaging agent (99m)Tc recombinant human Annexin V in human tumors after one course of chemotherapy as a predictor of tumor response and patient prognosis. Clin. Cancer Res. 8, 2766–2774. 35. , and Raghavan, D. (1997) Magnetic resonance imaging of response to chemotherapy in orthotopic xenografts of human bladder cancer. Clin. Cancer Res. 3, 1635–1641. 36. , et al. (2003) Chemosensitivity of peritoneal micrometastases as evaluated using green fluorescence protein (GFP)–tagged human gastric cancer cell line.
4. Preparation of Feeder Cells for Limiting Dilution Assay 1. 3 mL of tisue culture medium (see Note 7). 2. 6. 23 mL at –70°C. Use one tube for each experiment. 3. Incubate for 45 min at 37°C, with mixing every 15 min. 4. Pellet the cells and wash three times with 10 mL of tissue culture medium. 6 mL. 4. Notes 1. The fact that the cells are examined under a microscope can be considered an advantage of the cell-counting assay, because additional information is obtained. Heavily irradiated cells, which subsequently die, become very large.
Anticancer Drugs 10, 797–805. 43. , et al. (1998) Telomerase activity and telomere lengths in various cell lines: changes of telomerase activity can be another method for chemosensitivity evaluation. Int. J. Oncol. 13, 489–495. 44. , and Blumenthal, R. (2002) Sphingomyelin enhances chemotherapy efficacy and increases apoptosis in human colonic tumor xenografts. Int. J. Oncol. 20, 379–384. 45. Epstein, R. (1990) Drug-induced DNA damage and tumor chemosensitivity. J. Clin. Oncol. 8, 2062–2084. 46.
Chemosensitivity: Volume I: In Vitro Assays (Methods in Molecular Medicine) by Rosalyn D. Blumenthal