By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Ira H. Pastan
The seriously acclaimed laboratory usual, equipment in Enzymology, is among the so much hugely revered courses within the box of biochemistry. when you consider that 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this day - really an important book for researchers in all fields of existence sciences.Key positive aspects* Covers fundamentals in nice element* Laboratory necessities and Media phone CultureTechniques: Monolayers, development size, and Mass tradition
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Extra resources for Cell Culture
This is achieved as follows: Select cells in the logarithmic phase of growth and r e m o v e the culture medium. Assess sterility by culture on a blood agar plate. Replace with fresh m e d i u m and harvest 24 hr later for storage in liquid nitrogen. The cell culture m e d i u m should be the one which best supports growth and p r e s e r v a t i o n o f desired characteristics of the cell. Cell cultures grown in synthetic m e d i u m without s e r u m have been successfully frozen and recovered.
6 units/ml measured under the actual trypsinization conditions, s) Immediately remove as much as possible of the trypsin solution. Close the flask tightly to prevent evaporation and leave it on ice for about 5 min with only a thin film of trypsin solution covering the cells. Progress of the digestion may be checked by brief examination with an inverted microscope, but the flask should not be removed from the ice long enough to warm appreciably. When the cells are partially loosened, add 5 ml of chilled serum-free culture medium to the flask and shake s W.
6). 0 ml of twice-crystallized trypsin at a concentration of 100/zg/ml in solution I. 6 units/ml measured under the actual trypsinization conditions, s) Immediately remove as much as possible of the trypsin solution. Close the flask tightly to prevent evaporation and leave it on ice for about 5 min with only a thin film of trypsin solution covering the cells. Progress of the digestion may be checked by brief examination with an inverted microscope, but the flask should not be removed from the ice long enough to warm appreciably.
Cell Culture by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Ira H. Pastan