By David J. Sharp (eds.)
Mitosis: tools and Protocols offers state of the art overviews at the most crucial ways at present utilized in mitosis examine spanning from the research of unmarried molecules in isolation to their usage in the complicated surroundings of the phone. the quantity is split into 4 components, each one considering tools touching on targeted elements of mitosis examine. half I offers techniques for visualizing and examining the dynamic behaviors of the spindle gear, the microtubule established computer that drives chromosome segregation. half II focuses extra quite often on tools for learning and manipulating the microtubule cytoskeleton in cells and complicated telephone loose extracts. half III offers cutting-edge biophysical and excessive solution microscopy techniques for assessing advanced interactions among microtubules and microtubule-associated proteins in isolation in addition to microtubule constitution in cells. half IV presents equipment for learning the results of phone form on mobile department and strategies for quantifying aneuploidy (aberrant chromosome quantity) which often effects from mitotic defects and has been associated with human maladies starting from beginning defects to melanoma. Written within the hugely profitable Methods in Molecular Biology series structure, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, with no trouble reproducible laboratory protocols and key tips about troubleshooting and averting recognized pitfalls.
Authoritative and sensible, Mitosis: tools and Protocols seeks to supply different equipment and new innovations to deal with new or previous questions on the topic of the mechanisms of mitosis.
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