By Daniel Herschlag
This MIE quantity presents laboratory recommendations that goal to foretell the constitution of a protein that could have large implications starting from drug layout, to mobile pathways and their dynamics, to viral access into cells. specialist researchers introduce the main complicated applied sciences and strategies in protein constitution and foldingIncludes strategies on tiling assays.
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Additional resources for Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding
Integrins Edited by DAVID CHERESH VOLUME 427. MicroRNA Methods Edited by JOHN J. ROSSI VOLUME 428. Osmosensing and Osmosignaling Edited by HELMUT SIES AND DIETER HAUSSINGER VOLUME 429. Translation Initiation: Extract Systems and Molecular Genetics Edited by JON LORSCH VOLUME 430. Translation Initiation: Reconstituted Systems and Biophysical Methods Edited by JON LORSCH VOLUME 431. Translation Initiation: Cell Biology, High-Throughput and Chemical-Based Approaches Edited by JON LORSCH VOLUME 432.
General Considerations for Splinted RNA Ligation 3. 1. 2. Targeted RNase H cleavage of telomerase RNA 4. 1. Protocol 3: Dye-labeling and HPLC purification of synthetic RNA oligonucleotides 5. 1. Protocol 4: Splinted RNA ligation method for producing FRET-labeled telomerase RNA 6. Application: Single-Molecule FRET Measurements Acknowledgments References 28 29 31 31 33 37 39 40 42 44 45 45 Abstract Mechanistic studies of RNA enzymes (ribozymes) and ribonucleoprotein (RNP) complexes such as the ribosome and telomerase, often seek to characterize RNA structural features, either dynamic or static, and relate these properties to specific catalytic functions.
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15, 8783–8798. Pestova, T. , Shatsky, I. , Fletcher, S. , Jackson, R. , and Hellen, C. U. (1998). A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initiation of hepatitis C and classical swine fever virus RNAs. Genes Dev. 12, 67–83. , and Breaker, R. R. (2006). Characteristics of the glmS ribozyme suggest only structural roles for divalent metal ions.
Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding by Daniel Herschlag