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Download PDF by Marlene A. DeLuca (Eds.): Bioluminescence and Chemiluminescence

By Marlene A. DeLuca (Eds.)

ISBN-10: 0121819574

ISBN-13: 9780121819576

The seriously acclaimed laboratory general, Methods in Enzymology, is likely one of the so much hugely revered guides within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this day - actually a vital booklet for researchers in all fields of existence sciences

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8. Rapid kinetics of oxygen-pulsed ATP synthesis by sonicated inner membrane vesicles. Luminescence is measured vertically in units equivalent to 1 nmol of ATP per gram of protein. In (A), oxygen is pulsed to reduced vesicles with succinate as respiratory substrate. 4/~M ADP, 4 mg. 4, 23°. In (B), oxygen is pulsed to antimycin-inhibited and tetramethylphenylenediamine-bypassed sonicated inner membrane vesicles. Antimycin blocks site II of the respiratory chain. Tetramethylphenylenediamine is added in sufficient quantity to restore respiration to 20% of its original rate by bypassing the antimycin-inhibited site.

The fractionation of the firefly light-emitting system on various Sephadex gels is described by Nielsen and Rasmussen. 1 A purification of luciferase, almost as good as that described above, was obtained after 2 days' chromatography. 3, at 4° under nitrogen and in the dark. A preparation with very low adenylate kinase activity was obtained. The adsorption of luciferin and dehydroluciferin to low-porosity Sephadex gels is probably unique for this material. The data of Schram e t " W. D. McElroy and J.

Adenine nucleotide (AdN)-stimulated ATP production after KCN inhibition of respiration in mitochondria. Reaction medium is as described for Fig. 2. KCN (when added) is 2 raM. AdN (when added) is composed of 58/xM AMP and 15 g M ADP. The recording begins with the addition of mitochondria. the intensities of luminescence just before and just after the abrupt change in question. IS] is ATP concentration before the abrupt change and must be determined in a parallel experiment. In the example shown in Fig.

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Bioluminescence and Chemiluminescence by Marlene A. DeLuca (Eds.)

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