By Jose M Martinez-Zapater, Julio Salinas
This accomplished number of present and crucial protocols includes many simply reproducible equipment constructed to be used with Arabidopsis - a method for imminent primary questions in plant biology. The tools variety from the fundamentals of transforming into those crops to stylish gene cloning options and will, in lots of situations, even be utilized to different plant species with minor differences. Sections on genetics, transformation and gene expression research which are in particular important to scientists excited by mutant research or generating and reading transgenic crops.
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Additional info for Arabidopsis Protocols (Methods in Molecular Biology (Cloth))
SDS) and high concentration of EDTA. The isolation of plant DNA 1saffected by many factors. These include the amount and the type of tissues, species chosen, the number of steps involved, the time required to complete the procedure, the number of samples to be processed, the requisite purity of the extracted DNA for a specific appltcation, and the use of expensive and/or toxic chemicals. Of these factors the type of plant tissues IS the most Important one. The structure of the cell walls as well as From Methods m Molecular B/ology, Vol 82 Arabldopsls Protocols Edlted by J Martmez-Zapater and J Sahnas 0 Humana Press Inc , Totowa, 55 NJ Li and Chary 56 the molecular composltlon of the cytoplasm may influence the efficiency of a particular protocol.
And Reder, G P (1992) T-DNA transformation and msertlonal mutagenesrs, m Methods zn Arabtdopsrs Research (Koncz, C , Chua, N-H , and Schell, J , eds ), World Scientific, Singapore, pp. 224273 8 Mason, J. , Damm, B , Halfter, U , Wrllmttzer L , and Morns, P. ), World Screnttfic, Singapore, pp 310-330 Preparation of Physiologically from Arabidopsis Active Chloroplasts Ljerka Kunst 1. Introduction Only structurally intact chloroplasts with a functional envelope exhibit metabolic activities comparable to those of the original tissues.
New Phytol 75, l-l 0 6 Deheu, T , and Walker, D A ( 1972) An improved cathode for the measurement of the photosynthettc oxygen evolutton by isolated chloroplasts New Phytol. 71,201-225 8 Purification of Mitochondria from Arabidopsis Mathieu Klein, Stefan Binder, and Axe1 Brennicke 1. Introduction Plant cells contam different subcellular compartments, which serve distinct phystologrcal functions. One of these organelles, the mitochondrion, provides most of the nonphotosynthetrc energy required m the cell.
Arabidopsis Protocols (Methods in Molecular Biology (Cloth)) by Jose M Martinez-Zapater, Julio Salinas