By Richard J. Block, Emmett L. Durrum, Gunter Zweig
A handbook of Paper Chromatography and Paper Electrophoresis presents a complete dialogue of the innovations of paper chromatography and paper electrophoresis. The e-book is equipped into elements. half I on paper chromatography presents a with ease available resource for the various many makes use of and diversifications of paper chromatography. An attempt has been made to jot down a realistic guide during which attempted and proved tactics, making use of fairly basic gear and to be had reagents, are summarized. half II on paper electrophoresis discusses uncomplicated ideas and method. The emphasis all through has been at the separation of protein combos, relatively blood serum. This displays the truth that it truly is during this specific software that paper electrophoresis has up to now no longer been challenged through paper chromatography, while the various smaller molecules will be resolved both good or greater by way of the up to now extra largely hired chromatographic tactics.
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Extra info for A Manual of Paper Chromatography and Paper Electrophoresis
Other factors which apparently do not influence the R/ are: (a) equilibration of paper, (b) time of development, and (c) concentration of solutes (amino acids). These observations suggest that circular paper chromatography is a more rapid and simpler method than the previously described techniques. For a more detailed description of circular paper chromatography and its application to the resolution of a wide variety of compounds, the following additional papers are cited: Giri (1951, 1952, 1953a, 1953b), Rao (1952, 1953), Kariyone (1952), Lüderitz (1952a, 1952b), Zimmermann (1951), and Berlingozzi (1952).
A densitometer designed by Block (1950) consists of a barrier layer photocell, a suitable light source, and a sensitive galvanometer (Rubicon Spotlight No. 3402-HH or Pfaltz and Bauer Multiple Mirror Galvanometer No. 1810). When used on one-dimensional chromatograms, the filter paper strip may be pulled slowly across the beam of light, and the amount of transmittance is recorded on the galvanometer via the photocell. Figure 14 shows the light box in greater detail. The a-c line current (115 volts) is passed through a voltage regulator, adjusted by a coarse and fine variable resistance, and is reduced to 6 volts by a stepdown transformer.
Addendum APPLICATION OF SAMPLE The principal factor in obtaining reproducible results in paper chromatography from day to day and by different operators is believed to be the application of the spot by placing the micropipette at right angles to the paper (Livingston, 1953). * Another problem, that of concentrating an unknown dilute solution to be analyzed right on the filter paper, was solved as follows (Urbach, 1949) : Paper strips are stretched horizontally above a hot plate; both ends of the paper are weighted down by window display hooks.
A Manual of Paper Chromatography and Paper Electrophoresis by Richard J. Block, Emmett L. Durrum, Gunter Zweig